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Title Details:
Laboratory tests in clinical chemistry
Authors: Karkalousos, Petros
Zoi, Georgiou
Kroupis, Christos
Papaioannou, Angelos
Plageras, Panagiotis
Spyropoulos, Vasileios
Tsotsou, Georgia Eleni
Fountzoula, Christina
Reviewer: Karikas, Georgios-Alvertos
Subject: MEDICINE AND HEALTH SCIENCES, LIFE SCIENCES, BIOLOGICAL SCIENCES > LIFE SCIENCES > MOLECULAR BIOSCIENCES > BIOCHEMISTRY
Keywords:
Clinical chemistry
Photometry
Automatic analyzers
Statistical quality control
Immunochemistry
High performance liquid chromatography
Description:
Abstract:
This book aims to be a complete laboratory aid for students who study a clinical chemistry such as students in the departments of Biomedical Science, Chemistry, Biology and Medicine. The book covers a large variety of subjects such as biochemical analyses (substrates, enzymes, etc.), immunochemical analyses (hormones, cancer markers, etc.), electrolytes’ analyses and specialized techniques such as high-performance liquid chromatography, infrared spectroscopy and atomic absorption. The main purpose of his book is to cover: 1. The basic principles of clinical chemistry analyses: lambert-Beer law, properties of light, reference curves, types of biochemical analyses (endpoint, kinetic), basic principles of immunochemical analyses (ELISA), steps of biochemical analysis with manual and automate methods. Especially for classical clinical chemistry tests, a list and interpretation of the basic analytical methods will be given. Examples and exercises will be given for each type of analysis. 2. Modern analytical technology: a brief review of the history of the relevant technology from the photometer to the modern biochemical analyser will be given. We will describe the technologies in different types of analyzers (continuous flow, random access), the basic mechanical parts of biochemical and immunochemical analyzers as well as simple photometers, ion-selective electrodes, HPLC and atomic absorption analyzers. 3. Quality control: basic principles of statistical quality control, random and systematic errors, control charts, the difference between internal and external quality control, etc. will be described. 4. Laboratory safety 5. Additional chapters cover topics οf ISO 15189 for the accreditation of medical laboratories.
Linguistic Editors: Klada, Nektaria
Technical Editors: Stavroulakis, Peter
Graphic Editors: Laskari, Iro
Type: Laboratory Guide
Creation Date: 2015
Item Details:
ISBN 978-960-603-113-7
License: http://creativecommons.org/licenses/by-nc-nd/3.0/gr
DOI http://dx.doi.org/10.57713/kallipos-500
Handle http://hdl.handle.net/11419/5382
Bibliographic Reference: Karkalousos, P., Zoi, G., Kroupis, C., Papaioannou, A., Plageras, P., Spyropoulos, V., Tsotsou, G., & Fountzoula, C. (2015). Laboratory tests in clinical chemistry [Laboratory Guide]. Kallipos, Open Academic Editions. https://dx.doi.org/10.57713/kallipos-500
Language: Greek
Consists of:
1. Basic principles of photometry and colorimetric analyses
2. Chromatometric endpoint determinations. Examples from classical clinical chemistry in the determination of substrates. Calibration and technique
3. Chromatometric kinetic determinations. Examples from classical clinical chemistry in the determination of enzymes. Calibration and technique
4. Laboratory testing of acid-base balance and electrolytes
5. Electrophoresis. Basic principles - modern technology. Applications in clinical chemistry
6. Immunochemical determinations. Fundamentals of ELISA
7. Automated analyzers and modern biomedical technology in the clinical chemistry laboratory
8. High pressure (performance) liquid chromatography in clinical chemistry. Basic principles and examples
9. IVD products - enclosed leaflets of reagents and information they contain. The determination of reference values and other relevant requirements
10. Reference materials - traceability. Laboratory accreditation. The basics of uncertainty estimation
11. Quality control in modern clinical chemistry analyses
12. Atomic absorption and its applications
13. Infrared spectroscopy and its applications in clinical chemistry
14. Occupational health and safety in the clinical laboratory. Management of sanitary waste
Number of pages 407
Publication Origin: Kallipos, Open Academic Editions
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