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Title Details:
Laboratory Methods for Protein Analysis
Authors: Poulas, Konstantinos
Sideris, Sotirios
Reviewer: Lagoumintzis, Georgios
Subject: MEDICINE AND HEALTH SCIENCES, LIFE SCIENCES, BIOLOGICAL SCIENCES > LIFE SCIENCES > MOLECULAR BIOSCIENCES > BIOCHEMISTRY
Keywords:
Proteins
Electrophoresis
Chromatography
Description:
Abstract:
Biochemistry, a vital and integral subject, is a cornerstone in the curricula of numerous departments in the Schools of Applied Sciences and the Schools of Health Sciences. Students' foundational and practical training in biochemical topics not only paves the way for understanding intricate biological reactions but also plays a pivotal role in the consolidation of complex biological concepts. This ebook is dedicated to presenting the fundamental laboratory techniques for the analysis and separation of proteins, underscoring the significance of students' training in this area. Proteins constitute a remarkably heterogeneous class of biological macromolecules. Away from their natural environment, they show chemical instability, creating the need for special handling during their isolation and analysis. The rapid development of Recombinant DNA technology and the multitude of applications resulting from its use, as well as the evolution and refinement of analytical methods, make it necessary to know the basic principles of isolation and handling of biological samples containing protein sources. In this specific Laboratory Guide, a thorough approach is attempted to understand basic analytical techniques related to the fractionation of proteins in their solutions. An attempt is made to present classical analytical methods of qualitative and quantitative analysis and clarify their basic operating principles. Gel electrophoresis, gel exclusion chromatography, affinity chromatography, and ion exchange chromatography will be presented.
Linguistic Editors: Kalliaras, Dimitris
Type: Laboratory Guide
Creation Date: 2015
Item Details:
ISBN 978-960-603-126-7
License: http://creativecommons.org/licenses/by-nc-nd/3.0/gr
DOI http://dx.doi.org/10.57713/kallipos-765
Handle http://hdl.handle.net/11419/2578
Bibliographic Reference: Poulas, K., & Sideris, S. (2015). Laboratory Methods for Protein Analysis [Laboratory Guide]. Kallipos, Open Academic Editions. https://dx.doi.org/10.57713/kallipos-765
Language: Greek
Consists of:
1. Introduction - Handling of protein samples
2. Protein electrophoresis
3. Affinity chromatography
4. Ion exchange chromatography
5. Gel filtration chromatography (Size exclusion chromatography)
Number of pages 122
Publication Origin: Kallipos, Open Academic Editions
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