Title Details: | |
Ion exchange chromatography |
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Authors: |
Poulas, Konstantinos Sideris, Sotirios |
Reviewer: |
Lagoumintzis, Georgios |
Subject: | MEDICINE AND HEALTH SCIENCES, LIFE SCIENCES, BIOLOGICAL SCIENCES > LIFE SCIENCES > MOLECULAR BIOSCIENCES > BIOCHEMISTRY |
Description: | |
Abstract: |
In ion exchange chromatography, proteins can be separated based on their net electrical charge. Proteins with a net positive charge typically bind to a column filled with immobilized groups carrying negative charges, and vice versa. The release of protein fractions contained in the sample for analysis is achieved by gradually eluting them from the column using a specific elution buffer. The ions in the elution buffer compete with and displace components of the sample that are bound to the column with similar charges.
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Linguistic Editors: |
Kalliaras, Dimitris |
Type: |
Chapter |
Creation Date: | 2015 |
Item Details: | |
License: |
Attribution - NonCommercial - ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
Handle | http://hdl.handle.net/11419/2581 |
Bibliographic Reference: | Poulas, K., & Sideris, S. (2015). Ion exchange chromatography [Chapter]. In Poulas, K., & Sideris, S. 2015. Laboratory Methods for Protein Analysis [Laboratory Guide]. Kallipos, Open Academic Editions. https://hdl.handle.net/11419/2581 |
Language: |
Greek |
Is Part of: |
Laboratory Methods for Protein Analysis |
Number of pages |
17 |
Publication Origin: |
Kallipos, Open Academic Editions |