Title Details: | |
Gel filtration chromatography (Size exclusion chromatography) |
|
Authors: |
Poulas, Konstantinos Sideris, Sotirios |
Reviewer: |
Lagoumintzis, Georgios |
Subject: | MEDICINE AND HEALTH SCIENCES, LIFE SCIENCES, BIOLOGICAL SCIENCES > LIFE SCIENCES > MOLECULAR BIOSCIENCES > BIOCHEMISTRY |
Description: | |
Abstract: |
Size exclusion chromatography is a significant method for separating protein molecules based on their size. A mixture of molecules to be separated is loaded onto the top of a column filled with porous material, and the molecules move along the column either under gravity or with the assistance of an external force (pumping). The sample volume is kept as minimal as possible during this process. Molecules move through or between the beads of the packing material towards the bottom of the column according to their size. Larger molecules elute earlier and faster, as they pass through the void spaces between the beads, while smaller molecules traverse more convoluted paths through the packing material. This results in highly efficient separation.
|
Linguistic Editors: |
Kalliaras, Dimitris |
Type: |
Chapter |
Creation Date: | 2015 |
Item Details: | |
License: |
http://creativecommons.org/licenses/by-nc-nd/3.0/gr |
Handle | http://hdl.handle.net/11419/2582 |
Bibliographic Reference: | Poulas, K., & Sideris, S. (2015). Gel filtration chromatography (Size exclusion chromatography) [Chapter]. In Poulas, K., & Sideris, S. 2015. Laboratory Methods for Protein Analysis [Laboratory Guide]. Kallipos, Open Academic Editions. https://hdl.handle.net/11419/2582 |
Language: |
Greek |
Is Part of: |
Laboratory Methods for Protein Analysis |
Number of pages |
13 |
Publication Origin: |
Kallipos, Open Academic Editions |