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| Title Details: | |
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Reading the primary DNA sequence and characterization of mutations |
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| Authors: |
Papanikolaou, Georgios Palaiologou, Danai Lilakos, Konstantinos |
| Reviewer: |
Patrinos, Georgios |
| Description: | |
| Abstract: |
Theoretical part: Reading the primary DNA sequence (Sanger method). Presentation of next-generation sequencing (NGS) methods. Mutation and polymorphism. Types of mutations. Mutation nomenclature. Comparison of primary sequence reading results with reference sequences using computer software. Reading chromatograms. Use of the JEMBOSS software package. Practical part: 1) Reading chromatograms 2) Comparing results with a reference sequence using ClustalX software 3) Determining changes in the amino acid sequence of the encoded protein (examples of polymorphisms in the TAS2R38 gene) using JEMBOSS software 4) Mutation nomenclature.
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| Linguistic Editors: |
Pantos, Panagiotis |
| Type: |
Chapter |
| Creation Date: | 2015 |
| Item Details: | |
| License: |
http://creativecommons.org/licenses/by-nc-sa/3.0/gr |
| Handle | http://hdl.handle.net/11419/648 |
| Bibliographic Reference: | Papanikolaou, G., Palaiologou, D., & Lilakos, K. (2015). Reading the primary DNA sequence and characterization of mutations [Chapter]. In Papanikolaou, G., Palaiologou, D., Katsareli, E., Katsila, T., Tsarouha, H., Tzetis, M., Lilakos, K., & Doukissas, L. 2015. Laboratory Exercises in Human Genetics [Laboratory Guide]. Kallipos, Open Academic Editions. https://hdl.handle.net/11419/648 |
| Language: |
Greek |
| Consists of: |
1. Τριφωσφορικά 2’,3’–διδεοξυνουκλεοτίδια (ddNTPs) 2. Αποτελέσματα αλληλούχισης με τη μέθοδο κατά Sanger 3. Μέθοδος αλληλούχισης κατά Sanger. 4. Αλληλούχιση επόμενης γενεάς. 5. Βάθος αλληλούχισης. 6. Χρωματογράφημα αλληλούχισης (ετεροζυγωτία) |
| Is Part of: |
Laboratory Exercises in Human Genetics |
| Number of pages |
23 |
| Publication Origin: |
Kallipos, Open Academic Editions |