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| Title Details: | |
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Quality control and measurement of nucleic acid concentration |
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| Authors: |
Papanikolaou, Georgios Palaiologou, Danai |
| Reviewer: |
Patrinos, Georgios |
| Description: | |
| Abstract: |
This chapter reviews the methods of ultraviolet spectrophotometry, fluorometry, and electrophoresis for measuring the concentration and evaluating the purity and quality of isolated nucleic acids. In the practical part of the exercise, the concentration of DNA is determined, and a working solution of a specific concentration is prepared.
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| Linguistic Editors: |
Pantos, Panagiotis |
| Type: |
Chapter |
| Creation Date: | 2015 |
| Item Details: | |
| License: |
http://creativecommons.org/licenses/by-nc-sa/3.0/gr |
| Handle | http://hdl.handle.net/11419/646 |
| Bibliographic Reference: | Papanikolaou, G., & Palaiologou, D. (2015). Quality control and measurement of nucleic acid concentration [Chapter]. In Papanikolaou, G., Palaiologou, D., Katsareli, E., Katsila, T., Tsarouha, H., Tzetis, M., Lilakos, K., & Doukissas, L. 2015. Laboratory Exercises in Human Genetics [Laboratory Guide]. Kallipos, Open Academic Editions. https://hdl.handle.net/11419/646 |
| Language: |
Greek |
| Consists of: |
1. Η μείωση της ισχύος της διερχόμενης (Ι) μονοχρωματικής ακτινοβολίας εξαρτάται από τη συγκέντρωση της απορροφούσας ουσίας και τη διανυθείσα απόσταση εντός του διαλύματος. 2. Τυπική διάταξη των μερών ενός φασματοφωτόμετρου. 3. Φασματοφωτόμετρα μικρού όγκου 4. Ποσοτικοποίηση δειγμάτων DNA με χρήση φθορισμόμετρου. |
| Is Part of: |
Laboratory Exercises in Human Genetics |
| Number of pages |
11 |
| Typical Learning Time: |
PT01H00M00S |
| Publication Origin: |
Kallipos, Open Academic Editions |