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| Title Details: | |
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DNA isolation |
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| Authors: |
Papanikolaou, Georgios Palaiologou, Danai Katsareli, Efthymia |
| Reviewer: |
Patrinos, Georgios |
| Description: | |
| Abstract: |
The steps for DNA isolation include the lysis of cell membranes, degradation of proteins, separation of DNA from other cellular components, purification, and storage of DNA in an appropriate buffer solution. DNA is an excellent starting material for molecular biology experiments because it can be easily isolated from many sources, is extremely stable, and can be preserved for a long time under simple freezing conditions. In the experimental part, DNA is isolated from epithelial cells of the oral mucosa.
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| Linguistic Editors: |
Pantos, Panagiotis |
| Type: |
Chapter |
| Creation Date: | 2015 |
| Item Details: | |
| License: |
http://creativecommons.org/licenses/by-nc-sa/3.0/gr |
| Handle | http://hdl.handle.net/11419/645 |
| Bibliographic Reference: | Papanikolaou, G., Palaiologou, D., & Katsareli, E. (2015). DNA isolation [Chapter]. In Papanikolaou, G., Palaiologou, D., Katsareli, E., Katsila, T., Tsarouha, H., Tzetis, M., Lilakos, K., & Doukissas, L. 2015. Laboratory Exercises in Human Genetics [Laboratory Guide]. Kallipos, Open Academic Editions. https://hdl.handle.net/11419/645 |
| Language: |
Greek |
| Consists of: |
1. Το νουκλεόσωμα αποτελείται από μόρια DNA περιελιγμένα γύρω από ιστόνες. 2. Ο τρόπος δράσης του SDS. 3. Απεικόνιση των σταδίων απομόνωσης γενωμικού DNA με τη χρήση εμπορικά διαθέσιμων κιτ με στήλες που φέρουν μεμβράνη πυριτίου. 4. Απεικόνιση των σταδίων απομόνωσης γενωμικού DNA με τη χρήση μαγνητικών σφαιριδίων. |
| Is Part of: |
Laboratory Exercises in Human Genetics |
| Number of pages |
13 |
| Typical Learning Time: |
PT02H00M00S |
| Publication Origin: |
Kallipos, Open Academic Editions |