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Title Details:
Biological membranes: From structure to functions. Theoretical and experimental approaches
Authors: Lekka, Maria Eleni E
Leondaritis, George
Galanopoulou, Konstantina
Kitsiouli, Eirini
Reviewer: Kyriakidis, Dimitrios
Subject: NATURAL SCIENCES AND AGRICULTURAL SCIENCES > CHEMISTRY > BIOCHEMISTRY
NATURAL SCIENCES AND AGRICULTURAL SCIENCES > CHEMISTRY > BIOCHEMISTRY > MEMBRANES
Keywords:
Biological membranes
Membrane lipids
Membrane proteins
Membrane transport
Signal transduction
Bioinformatics
Membrane lab exercises
Description:
Abstract:
"Biological membranes: From structure to functions. Theoretical and experimental approaches" is a textbook exploring the main concepts of the structure and functions of biological membranes, at a theoretical and practical level. The sequence of the theoretical chapters follows a hierarchical logic, aspiring to stick to definitions, assumptions and methodology. We start with the main types of eukaryotic and prokaryotic cells' biological membranes, and we discuss techniques for cell homogenisation, fractionation and purification based on enzymic and morphological purity criteria. Chemical and physicochemical properties of the membrane components follow, solubilization and reconstitution techniques, while main concepts regarding Critical Micellar Concentration, and hydrophobic-hydrophilic balance, are described. Then, we proceed to the membrane components' assembling without disturbing the cellular integrity, dynamics and membrane biogenesis, procedures leading to the architecture of biological membranes. Models of membrane structure, the fluid mosaic model, assumptions, consistencies and inconsistencies of the models and applications regarding synthetic membranes and liposomes are comprised. The chapter devoted to transport explains how hydrophobic and hydrophilic compounds pass through highly specific channels and describes primary and secondary active transport and the transport of particles through whole regions of biological membranes often in the form of endocytosis, via receptors. At the level of membranes' functions, we provide the fundamentals and logic of cell communication and signalling. Types of membrane receptors, classification and kinetics are outlined, followed by classical examples of signalling pathway patterns, such as G-proteins, effectors and intracellular messenger systems. Among the plethora of pathways, we focus on lipid signalling through intracellular and extracellular lipid signalling molecules. Finally, 10 classical experiments and techniques for the analysis of biological membranes are presented. In this respect, the most common techniques applying to the study of biological membranes and membrane components are presented. All the lab exercises contain relevant comprehensive technical appendixes.
Technical Editors: Velivasi, Glykeria
Graphic Editors: Dedikousi, Stamatia
Type: Undergraduate textbook
Creation Date: 2015
Item Details:
ISBN 978-960-603-387-2
License: http://creativecommons.org/licenses/by-nc-nd/3.0/gr
DOI http://dx.doi.org/10.57713/kallipos-590
Handle http://hdl.handle.net/11419/4307
Bibliographic Reference: Lekka, M., Leondaritis, G., Galanopoulou, K., & Kitsiouli, E. (2015). Biological membranes: From structure to functions. Theoretical and experimental approaches [Undergraduate textbook]. Kallipos, Open Academic Editions. https://dx.doi.org/10.57713/kallipos-590
Language: Greek
Consists of:
1. Cell membranes: Classification and separation techniques
2. Chemical composition of biological membranes
3. Structure of biological membranes
4. Architecture and assembly of biological membranes
5. Membrane transport
6. Guiding signal transduction principals. Membrane receptors
7. Cell cultures, Cell activation by LPS
8. SDS-Polyacrylamide Gel Electrophoresis
9. Protein transport and sPLA2-IIA detection
10. RT-qPCR of sPLA2-IIA
11. Investigation of protein structure through Cn3D programme
12. Apoptosis assessment using flow cytometry
13. Protein immunofluorescence in lipid localisation. Applications in phosphatidylinositol signalling
14. Enzymic assay of PTEN
15. Enzymic assay of PLC
16. Enzymic assay of PLD in plants
17. Membrane trafficking. A microspopical study of phagocytosis in Tetrahymena thermophyla
18. Isolation, quantitative determination and characterisation of phospholipids from Tetrahymena thermophyla
19. Photometric determination of total phospholipid esters
20. Gas chromatographic analysis of fatty acid methylesters from phospholipids
21. Isolation and purification of prostaglandin G/H2 synthase from A549 cells
Number of pages 344
Publication Origin: Kallipos, Open Academic Editions
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